Ordering

This material is available to academics and nonprofits only.

Backbone

• Vector backbone
  pDONR221
  (Search Vector Database)
• Backbone manufacturer
  Invitrogen
• Backbone sizew/o insert(bp)2538
• Vector type
  Mammalian Expression, Mouse Targeting, Cre/Lox
• Selectable markers
  Hygromycin

Growth in Bacteria

• Bacterial Resistance(s)
  Kanamycin
• Growth Temperature
  37°C
• Growth Strain(s)
  DH5alpha
• Copy number
  High Copy

Gene/Insert

• Gene/Insert name
  Split GFP-RFP chimera
• Species
  Aequorea victoria and Discosoma sp. reef coral
• Insert Size (bp)
  3948

Cloning Information

• Cloning methodGateway Cloning
• 5′ cloning siteattL1(not destroyed)
• 3′ cloning siteattL2(not destroyed)
• 5′ sequencing primerM13 forward
• 3′ sequencing primerM13 reverse
(Common Sequencing Primers)

Resource Information

• Terms and Licenses
  • UBMTA
  • Ancillary Agreement for Plasmids Containing FP Materials
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms
  • Not Available to Industry