Ordering

This material is available to academics and nonprofits only.

Backbone

• Vector backbone
  pLVTHM
  (Search Vector Database)
• Backbone manufacturer
  Trono Laboratory
• Backbone sizew/o insert(bp)11085
• Total vector size (bp)12000
• Vector type
  Mammalian Expression, Lentiviral, RNAi

Growth in Bacteria

• Bacterial Resistance(s)
  Ampicillin
• Growth Temperature
  37°C
• Growth Strain(s)
  Stbl3
• Growth instructions
  Use Stbl3 or HB101 to reduce chance of recombination. Grow at 37C.
• Copy number
  High Copy

Gene/Insert

• Gene/Insert name
  shREV3-4
• Alt name
  REV3L
• gRNA/shRNA sequence
  CAAAGATGCTGCTACATTA
• Species
  H. sapiens (human), M. musculus (mouse), R. norvegicus (rat); human
• GenBank ID
  1.NM_002912.3
• Entrez Gene
  Rev3l(a.k.a. Rev, Rev3, Sez4)
• Entrez Gene
  Rev3l
• Entrez Gene
  REV3L(a.k.a. POLZ, REV3)

Cloning Information

• Cloning methodRestriction Enzyme
• 5′ cloning siteEcoRI(not destroyed)
• 3′ cloning siteClaI(not destroyed)
• 5′ sequencing primer?
• 3′ sequencing primer?
(Common Sequencing Primers)

Resource Information

• Terms and Licenses
  • UBMTA
• Industry Terms
  • Not Available to Industry

Depositor Comments