Ordering

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Backbone

• Vector backbone
  pZ with ColE origin
  (Search Vector Database)
• Backbone manufacturer
  Lim Lab
• Modifications to backbone
  Plasmid modified from Rolf Lutz and Hermann Bujard, 1997
• Vector type
  Bacterial Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s)
  Ampicillin
• Growth Temperature
  37°C
• Growth Strain(s)
  XL1 Blue
• Copy number
  High Copy

Gene/Insert1

• Gene/Insert name
  GFP
• Alt name
  green fluorescent protein
• Insert Size (bp)
  717
• PromoterpConM12NoHind
• Tags/ Fusion Proteins
  • ASV degradation tag (C terminal on backbone)
  • glgC leader region (N terminal on backbone)

Cloning Informationfor Gene/Insert 1

• Cloning methodRestriction Enzyme
• 5′ cloning siteSphI(not destroyed)
• 3′ cloning siteNheI(not destroyed)
• 5′ sequencing primerGCTGGGATTA CACATGGCAT GGAT
• 3′ sequencing primercgcggatcc AAGGCCATCCGTCAGGATGGCCTT
(Common Sequencing Primers)

Gene/Insert2

• Gene/Insert name
  TetR
• PromoterpConNoHind

Cloning Informationfor Gene/Insert 2

• Cloning methodRestriction Enzyme
• 5′ cloning siteXmaI(not destroyed)
• 3′ cloning siteHindIII(not destroyed)
• 5′ sequencing primerGCTGGGATTA CACATGGCAT GGAT
• 3′ sequencing primerccggaattc ACTGCTCACA AGAAAAAAGG CACG
(Common Sequencing Primers)

Gene/Insert3

• Gene/Insert name
  CsrA
• Insert Size (bp)
  186
• Entrez Gene
  csrA(a.k.a. b2696, ECK2691, zfiA)
• PromoterpConNoHind

Cloning Informationfor Gene/Insert 3

• Cloning methodRestriction Enzyme
• 5′ cloning siteNotI(not destroyed)
• 3′ cloning siteApaI(not destroyed)
• 5′ sequencing primerggccaag CTT TAATC GTACAGGGTA GTACAAATA
• 3′ sequencing primerAGCTGATACC GCTCGCCGCA GCCGAACG
(Common Sequencing Primers)

Resource Information

• Terms and Licenses
  • UBMTA
  • Ancillary Agreement for Plasmids Containing FP Materials
• Industry Terms
  • Not Available to Industry