Addgene/pSicoR-shS6K1/2/1单元/81088
商品编号：
81088
品牌：
Addgene inc
市场价：
¥1500.00
美元价：
750.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | ||
|---|---|---|---|---|---|---|
| Plasmid | 81088 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $75 | Add to Cart | |
This material is available to academics and nonprofits only.
Backbone
- Vector backbonepSicoR(Search Vector Database)
- Backbone manufacturerJacks Lab (MIT)
- Backbone sizew/o insert(bp)7567
- Total vector size (bp)7609
- Vector typeMammalian Expression, Lentiviral, RNAi, Cre/Lox
Growth in Bacteria
- Bacterial Resistance(s)Ampicillin
- Growth Temperature37°C
- Growth Strain(s)NEB Stable
- Copy numberHigh Copy
Gene/Insert
- Gene/Insert nameS6K1 & S6K2 shRNA
- gRNA/shRNA sequenceCTCAGTGAGAGTGCCAACCAA
- SpeciesM. musculus (mouse)
- PromoterU6
Cloning Information
- Cloning methodRestriction Enzyme
- 5′ cloning siteHpaI(destroyed during cloning)
- 3′ cloning siteXhoI(not destroyed)
- 5′ sequencing primermU6-F (Common Sequencing Primers)
Resource Information
- A portion of this plasmid was derived from a plasmid made bypSicoR was obtained from Addgene (plasmid 11579). shRNA insert was synthesized independently but is based on a target sequence from Bae et al. 2012 (PMID: 22493495)
- Terms and Licenses
- UBMTA
- Industry Terms
- Not Available to Industry
Depositor Comments
pSicoR allows for expression of the S6K1/2 shRNA in the absence of Cre. Expression of Cre turns off shRNA expression. These plasmids were also used in the following publication "Normalizing translation through 4E-BP prevents mTOR-driven cortical mislamination and ameliorates aberrant neuron integration. Lin TV, Hsieh L, Kimura T, Malone TJ, Bordey A. Proc Natl Acad Sci U S A. 2016 Oct 4;113(40):11330-11335."
品牌介绍
Addgene腺相关病毒（AAV）是最初被发现为腺病毒原种污染物的小型病毒。使用AAV进行研究的一个主要优点是它受复制限制，通常不引起人类疾病。由于这些原因，AAV通常以较低的生物安全水平被包含并且在体内引起相对较低的免疫学作用。虽然AAV可以在BSL-1处理，但表达癌基因或毒素的AAV应该在BSL-2处理。AAV可以以低免疫应答和低毒性转导分裂和非分裂细胞。尽管重组AAV不能整合到宿主基因组中，但转基因表达可以长期存在。目前，AAV的实用性受到其小包装容量（包括ITR在内约4.5 kb）的限制，尽管有很多兴趣和精力来扩大这种容量。传统上，AAV需要存在另一种“辅助”病毒（例如腺病毒或疱疹病毒）才能传播。这是由于AAV对某些介导AAV复制的外源基因产物的依赖。“无辅助病毒的系统”已经绕过了这一要求，该系统无需使用辅助病毒就可以生产感染性AAV颗粒。替代地，可以在AAV生产过程中通过辅助质粒（例如pHelper）和特异性包装细胞系（例如HEK293细胞）提供特异性基因产物。
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