Ordering

This material is available to academics and nonprofits only.

Backbone

• Vector backbone
  pLenti4-V5/DEST
  (Search Vector Database)
• Backbone manufacturer
  Invitrogen
• Backbone sizew/o insert(bp)6949
• Total vector size (bp)9200
• Vector type
  Mammalian Expression, Lentiviral
• Selectable markers
  Zeocin

Growth in Bacteria

• Bacterial Resistance(s)
  Ampicillin
• Growth Temperature
  37°C
• Growth Strain(s)
  Stbl3
• Copy number
  High Copy

Gene/Insert

• Gene/Insert name
  P85(K550_K562dup)
• Alt name
  PIK3R1
• Species
  H. sapiens (human)
• Insert Size (bp)
  2175
• Mutation
  created internal duplication of amino acids 550-562
• Entrez Gene
  PIK3R1(a.k.a. AGM7, GRB1, IMD36, p85, p85-ALPHA)
• PromoterCMV
• Tag/ Fusion Protein
  • Flag (N terminal on insert)

Cloning Information

• Cloning methodGateway Cloning
• 5′ sequencing primerCMV forward
• 3′ sequencing primerV5 reverse
(Common Sequencing Primers)

Resource Information

• A portion of this plasmid was derived from a plasmid made by
  Flag-tagged human P85 construct was provided by Dr Jayne Stommel (National Cancer Institute).
• Terms and Licenses
  • UBMTA
• Industry Terms
  • Not Available to Industry