Ordering

This material is available to academics and nonprofits only.

Backbone

• Vector backbone
  pEZ-Frt-lox-DT
  (Search Vector Database)
• Backbone manufacturer
  Klaus Rajewsky (Addgene plasmid # 11736)
• Backbone sizew/o insert(bp)4500
• Modifications to backbone
  The FRT-flanked cassette in pEZ was replaced by a synthetic fragment bearing an FRT-flanked splicing acceptor together with multiple cloning sites. A Neo-Ires-GFP-SV40 polyA fragment was inserted immediately downstream of the splicing acceptor to create the gene-trapping cassette. The left and right arms flanking the cassette, encompassing BAF57 exons 2 to 3 and exon 4, respectively, were amplified by PCR from C57B/6 mice
• Vector type
  Cre/Lox
• Selectable markers
  Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s)
  Ampicillin
• Growth Temperature
  37°C
• Growth Strain(s)
  Stbl2
• Copy number
  Unknown

Gene/Insert

• Gene/Insert name
  promoter-trap cassette Neo-Ires-GFP

Cloning Information

• Cloning methodUnknown
• 5′ sequencing primerT3
• 3′ sequencing primerSV40pA-R
(Common Sequencing Primers)

Resource Information

• Terms and Licenses
  • UBMTA
  • Ancillary Agreement for Plasmids Containing FP Materials
• Industry Terms
  • Not Available to Industry

Depositor Comments