Ordering

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Backbone

• Vector backbone
  pLPC
  (Search Vector Database)
• Total vector size (bp)7051
• Vector type
  Mammalian Expression, Retroviral
• Selectable markers
  Puromycin

Growth in Bacteria

• Bacterial Resistance(s)
  Ampicillin
• Growth Temperature
  37°C
• Growth Strain(s)
  DH5alpha
• Copy number
  Unknown

Gene/Insert

• Gene/Insert name
  chimeric TRF
• Species
  H. sapiens (human)
• Insert Size (bp)
  1515
• Mutation
  TRF2 where TRFH domain (aa42-241) has been replaced by that of TRF1 (aa58-262); A434 deleted
• GenBank ID
  NP_005643.1 for TRF2NP_059523.2 for TRF1
• Entrez Gene
  TERF2(a.k.a. TRBF2, TRF2)
• Entrez Gene
  TERF1(a.k.a. PIN2, TRBF1, TRF, TRF1, hTRF1-AS, t-TRF1)
• Tag/ Fusion Protein
  • Myc (N terminal on insert)

Cloning Information

• Cloning methodRestriction Enzyme
• 5′ cloning siteBamHI(not destroyed)
• 3′ cloning siteEcoRI(not destroyed)
• 5′ sequencing primerpLPC-5
• 3′ sequencing primerpLPC-3
(Common Sequencing Primers)

Resource Information

• Terms and Licenses
  • UBMTA
• Industry Terms
  • Not Available to Industry
• Articles Citing this Plasmid
  • 3 References

Depositor Comments