Ordering

This material is available to academics and nonprofits only.

Backbone

• Vector backbone
  pRRLSIN.cppt.PGK-GFP.WPRE Addgene 12252
  (Search Vector Database)
• Backbone manufacturer
  Didier Trono
• Backbone sizew/o insert(bp)6857
• Total vector size (bp)8524
• Modifications to backbone
  PGK GFP removed (EcoRV and BamHI)and replaced with a multiple cloning site for addition of promoter CDS cassettes
• Vector type
  Lentiviral

Growth in Bacteria

• Bacterial Resistance(s)
  Ampicillin
• Growth Temperature
  37°C
• Growth Strain(s)
  Stbl3
• Copy number
  Low Copy

Gene/Insert

• Gene/Insert name
  GFP
• Alt name
  EGFP
• Alt name
  enhanced green fluorescent protein
• Insert Size (bp)
  720
• Entrez Gene
  eGFP(a.k.a. pPRS3a_01)
• PromoterWAS (human 1.6 kb)

Cloning Information

• Cloning methodRestriction Enzyme
• 5′ cloning siteMluI(not destroyed)
• 3′ cloning siteSpeI(not destroyed)
• 5′ sequencing primergatcacgagactagcctcgag
• 3′ sequencing primercaacgggccacaactcctc
(Common Sequencing Primers)

Resource Information

• A portion of this plasmid was derived from a plasmid made by
  Backbone is from Addgene plasmid 12252 (Didier Trono)
• Terms and Licenses
  • UBMTA
  • Ancillary Agreement for Plasmids Containing FP Materials
• Industry Terms
  • Not Available to Industry