Ordering

This material is available to academics and nonprofits only.

Backbone

• Vector backbone
  MDH1-PGK-GFP_2.0
  (Search Vector Database)
• Backbone manufacturer
  Addgene Plasmid Repository
• Backbone sizew/o insert(bp)6963
• Vector type
  Mammalian Expression, Retroviral, RNAi

Growth in Bacteria

• Bacterial Resistance(s)
  Ampicillin
• Growth Temperature
  37°C
• Growth Strain(s)
  DH5alpha
• Copy number
  High Copy

Gene/Insert

• Gene/Insert name
  transactivating protein
• Alt name
  tat; p14
• Species
  Human Immunodeficiency Virus
• Insert Size (bp)
  258
• Mutation
  From the "A" HIV clade
• GenBank ID
  155871
• Entrez Gene
  tat(a.k.a. HIV1gp5)
• Tag/ Fusion Protein
  • Flag (N terminal on insert)

Cloning Information

• Cloning methodRestriction Enzyme
• 5′ cloning siteNotI(not destroyed)
• 3′ cloning siteSalI(not destroyed)
• 5′ sequencing primermPGK fwd
• 3′ sequencing primerH1 primer
(Common Sequencing Primers)

Resource Information

• Terms and Licenses
  • UBMTA
• Industry Terms
  • Not Available to Industry