Ordering

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Backbone

• Vector backbone
  pLenti6.3/TO/V5-DEST
  (Search Vector Database)
• Backbone manufacturer
  invitrogen
• Backbone sizew/o insert(bp)9351
• Total vector size (bp)9185
• Modifications to backbone
  lox-dsRED-STOP-lox-GFP (from plasmid(Plasmid #32702)) cloned in to pLenti 6.3(through in-fusion cloning, linearized through PstI and SalI digestion, restriction sites maintained)
• Vector type
  Mammalian Expression, Lentiviral, Cre/Lox
• Selectable markers
  Blasticidin

Growth in Bacteria

• Bacterial Resistance(s)
  Ampicillin
• Growth Temperature
  37°C
• Growth Strain(s)
  NEB Stable
• Copy number
  Unknown

Gene/Insert1

• Gene/Insert name
  dsRED-express2
• Species
  Synthetic
• Insert Size (bp)
  678
• PromoterCMV

Cloning Informationfor Gene/Insert 1

• Cloning methodLigation Independent Cloning
• 5′ sequencing primerCGCAAATGGGCGGTAGGCGTG
• 3′ sequencing primerGTACTGGAACTGGGGGGACAG
(Common Sequencing Primers)

Gene/Insert2

• Gene/Insert name
  eGFP
• Species
  Synthetic
• Insert Size (bp)
  717
• PromoterCMV

Cloning Informationfor Gene/Insert 2

• Cloning methodLigation Independent Cloning
• 5′ sequencing primerGGTCCTTCTTGAGTTTGTAAC
• 3′ sequencing primerCCATCTAATTCAACAAGAATTGGGACAAC
(Common Sequencing Primers)

Resource Information

• A portion of this plasmid was derived from a plasmid made by
  insert derived from plasmid (Plasmid #32702) pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE
• Terms and Licenses
  • UBMTA
  • Ancillary Agreement for Plasmids Containing FP Materials
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms
  • Not Available to Industry