Ordering

This material is available to academics and nonprofits only.

Backbone

• Vector backbone
  pIS1
  (Search Vector Database)
• Backbone manufacturer
  Available from Addgene (#12179)
• Backbone sizew/o insert(bp)4085
• Vector type
  Mammalian Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s)
  Ampicillin
• Growth Temperature
  37°C
• Growth Strain(s)
  DH5alpha
• Copy number
  High Copy

Gene/Insert

• Gene/Insert name
  Dicer 1
• Alt name
  CM12
• Species
  H. sapiens (human)
• Insert Size (bp)
  4200
• Mutation
  Mutated let-7 and miR103/107 microRNA binding sites
• Entrez Gene
  DICER1(a.k.a. DCR1, Dicer, Dicer1e, GLOW, HERNA, K12H4.8-LIKE, MNG1, RMSE2)
• Tag/ Fusion Protein
  • Luciferase (N terminal on backbone)

Cloning Information

• Cloning methodRestriction Enzyme
• 5′ cloning siteXba I(not destroyed)
• 3′ cloning siteNot I(not destroyed)
• 5′ sequencing primern/a
• 3′ sequencing primerEBV Reverse
(Common Sequencing Primers)

Resource Information

• Terms and Licenses
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms
  • Not Available to Industry

Depositor Comments